Advances in renal (patho)physiology using multiphoton microscopy
نویسندگان
چکیده
منابع مشابه
Advances in renal (patho)physiology using multiphoton microscopy.
Multiphoton excitation fluorescence microscopy is a state-of-the-art confocal imaging technique ideal for deep optical sectioning of living tissues. It is capable of performing ultrasensitive, quantitative imaging of organ functions in health and disease with high spatial and temporal resolution which other imaging modalities cannot achieve. For more than a decade, multiphoton microscopy has be...
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Recent advances in microscopy and optics, computer sciences, and the available fluorophores used to label molecules of interest have empowered investigators to utilize intravital two-photon microscopy to study the dynamic events within the functioning kidney. This emerging technique enables investigators to follow functional and structural alterations with subcellular resolution within the same...
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Multi-Photon Fluorescence Microscopy is a relatively novel imaging technique in cell biology. It relies on the quasi-simultaneous absorption of two or more photons (of either the same or different energy) by a molecule. With normal fluorescence microscopy, one cannot resolve deep structures within a specimen because of large depth of focus. An enhanced method, the confocal microscopy [1], allow...
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Multiphoton imaging of the sub-cellular distribution of photosensitizers can provide important clues to their mechanism of action in tumors. We have used this tool to study distribution and pharmacology of photosensitizers in murine hepatoma tumor cells dosed with various photosensitizers. Upon photoactivation, Rose Bengal yields nearly immediate photolytic release of lysosomal enzymes, resulti...
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At the time of diagnosis, approximately 75% of bladder cancers are non-muscle invasive. Appropriate diagnosis and surgical resection at this stage improves prognosis dramatically. However, these lesions, being small and/or flat, are often missed by conventional white-light cystoscopes. Furthermore, it is difficult to assess the surgical margin for negativity using conventional cystoscopes. Resu...
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ژورنال
عنوان ژورنال: Kidney International
سال: 2007
ISSN: 0085-2538
DOI: 10.1038/sj.ki.5002461